Transcriptomic Profiling of Human Intestinal Cells Under Substrate and Shear Stress

A 2026 study by Nadra Alzain investigates the response of human intestinal epithelial cells to biomimetic substrates and shear stress using bulk RNA sequencing. The dataset, shared under a CC-BY-4.0 license, contains transcriptomic data from cells cultured on decellularized intestinal submucosa hydrogels in a custom millifluidic system. It reveals gene expression changes related to cell survival, differentiation, and metabolic pathway activation under dynamic culture conditions.

Use Cases

• Analyze gene expression signatures for absorptive lineage maturation based on transcriptomic profiling.
• Model transcriptional adaptation to mechanical shear stress using data from dynamic culture conditions.
• Compare cell differentiation and survival signaling on biomimetic dSIS-MA hydrogels versus standard substrates.
• Study the activation of metabolic and immune pathways in intestinal epithelial cells under defined biochemical cues.

Strengths

• Data is derived from extensive bulk RNA sequencing experiments, as stated in the description.
• Dataset has a specific temporal marker, last updated on 2026 - 05 - 01.
• Shared under a permissive CC - BY - 4.0 license for reuse.

Limitations

• Dataset scale is limited at 128.8 KB, suggesting a small scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare, author Nadra Alzain.

Collection Method

Bulk RNA sequencing of primary human intestinal epithelial cells cultured on 3D-printed hydrogels in a millifluidic system.

Time Range

Study published in 2026.

Freshness

Last updated 2026-05-01 04:16:29.

Geography

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