Groundcherry Gene Editing Results via Virus-Induced CRISPR-Cas9

This dataset documents the outcomes of a virus-induced gene editing (VIGE) experiment in groundcherry (Physalis grisea), targeting the PDS and CLV1 genes. It reports somatic editing frequencies of 80–95% for PDS and up to 73% for CLV1, with heritable edits observed in progeny.

Use Cases

• Analyze the relationship between somatic editing frequencies (80-95% for PDS, up to 73% for CLV1) and the recovery of heritable edits in progeny.
• Study the phenotypic outcomes, such as albino seedlings from PDS edits or increased floral organ number from CLV1 edits, resulting from specific gene mutations.
• Investigate the efficiency of generating mono-, bi-, tri-, and tetra-allelic mutations in a tetraploid plant line using the VIGE method.

Strengths

• Reports specific, high somatic editing frequencies (80–95% for PDS, up to 73% for CLV1).
• Demonstrates heritable gene edits, with all infected plants (n=5) producing edited progeny for PDS.
• Documents editing outcomes in a tetraploid context, showing robust editing across four alleles.

Limitations

• The primary data is embedded in a 35.9 KB PDF, requiring extraction for computational analysis.
• The sample size for the experiment is small, with n=5 plants reported for the PDS heritability assessment.
• The dataset is static, representing results from a single study without ongoing updates.

Provenance

Source

Redeat Tibebu via figshare.

Collection Method

Experimental results from Tobacco rattle virus (TRV)–mediated delivery of guide RNAs into Cas9-expressing groundcherry plants.

Freshness

Last updated 2026-03-20.