Vitamin D Metabolite Effects on Bovine Mammary Epithelial Cells and Mastitis Pathogens

A proteomic dataset from an in vitro study comparing the effects of three vitamin D3 metabolites on bovine mammary epithelial cells. The data includes results from antimicrobial assays, transcriptional regulation analysis, and proteomic profiling of 2,203 proteins common to all treatment conditions. The dataset was published by Georgina Tiraboschi on figshare under a CC-BY-4.0 license in May 2026.

Use Cases

• Compare host-directed versus direct antimicrobial effects of vitamin D metabolites based on the described in vitro assays.
• Analyze proteomic signatures related to epithelial homeostasis and innate immunity based on the 2,203 identified proteins.
• Investigate the regulation of vitamin D metabolism enzymes and receptors in mammary epithelial cells based on the transcriptional analysis.
• Model the reduction of Staphylococcus aureus internalization in pretreated cells based on the described experimental results.

Strengths

• Proteomic profiling identified 2,203 proteins common to all experimental conditions.
• The study design includes comparative analysis of three distinct vitamin D3 metabolites (D, 25D, and 1,25D).
• Data is published under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small in scale (166.9 KB), indicating limited data volume.

Provenance

Source

figshare

Collection Method

Integrated in vitro approach using bovine mammary epithelial MAC-T cells and mastitis-associated Staphylococcus spp. isolates.

Freshness

Last updated 2026-05-01 05:27:44; freshness should be verified.