TNBC Immune Microenvironment Data on 25HC Regulation of Macrophages

A figshare-hosted research document details the role of the oxysterol metabolite 25-hydroxycholesterol in the tumor immune microenvironment of triple-negative breast cancer. The study, authored by Hongmei Zheng and last updated in May 2026, employed multiplex fluorescence immunohistochemistry and bioinformatic analyses to identify 25HC as a key regulator inhibiting CD163+ macrophage polarization. These findings suggest a novel immunometabolic mechanism underlying TNBC aggressiveness.

Use Cases

• Study the link between lipid metabolism and immune cell function in cancer based on the identified 25HC regulatory axis.
• Validate bioinformatic predictions of tumor microenvironment composition using in vitro and in vivo models described in the methods.
• Investigate potential therapeutic targets for TNBC based on the dysregulated interferon regulatory factor 7–cholesterol-25-hydroxylase–25HC pathway.

Strengths

• The document is openly licensed under CC-BY-4.0, permitting reuse and redistribution.
• The research integrates multiple methodologies, including bioinformatics, immunohistochemistry, and cellular assays, to validate findings.

Limitations

• The dataset is a 55.1 KB DOCX file, suggesting it is a research paper or summary rather than raw structured data.
• Column-level documentation and sample data are unavailable, limiting immediate analysis.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Multiplex fluorescence immunohistochemistry and bioinformatic analyses, validated with in vitro cellular assays and in vivo mouse models.

Freshness

Last updated 2026-05-28 04:43:30; freshness should be verified.