Mouse Gut Microbiota Composition After Acute High-Intensity Exercise

Ruolin Gao's study on figshare, last updated April 22, 2026, investigates dynamic changes in gut microbiota following acute high-intensity exercise in BALB/c and C57BL/6 mouse strains. The dataset, 20.8 KB in size, includes results from 16S rRNA gene sequencing of colonic content samples collected at 0, 30, and 60 minutes post-exercise. It captures strain-specific microbial diversity and functional responses related to energy metabolism and gut integrity.

Use Cases

• Analyzing strain-specific gut microbiota diversity changes based on alpha and beta diversity metrics mentioned in the results.
• Investigating the relationship between exercise and energy metabolism based on the functional analysis of upregulated enzymes.
• Studying temporal dynamics of microbial composition based on sampling at three post-exercise time points.
• Identifying key exercise-responsive taxa like Muribaculum based on the genus-level analysis described.

Strengths

• Data includes temporal dynamics with samples collected at 0, 30, and 60 minutes post-exercise.
• Compares two distinct mouse strains (BALB/c and C57BL/6), allowing for strain-specific analysis.
• Results include specific statistical findings, such as p-values for changes in the Bacteroidetes/Firmicutes ratio.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (20.8 KB), indicating limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

Ruolin Gao via figshare.

Collection Method

16S rRNA gene sequencing of colonic content from mice after a 30-minute high-intensity treadmill run.

Time Range

Samples collected immediately, 30 minutes, and 60 minutes post-exercise.

Freshness

Last updated 2026-04-22 16:31:40; freshness should be verified.

Geography

Laboratory study using mouse models; specific geographic location is unknown.