Characterization of Novel Spectinomycin Resistance Gene ANT(9)-If

Molecular characterization data for the ant(9)-If gene, a novel plasmid-encoded aminoglycoside adenylyltransferase. The dataset includes enzyme kinetic parameters and comparative minimum inhibitory concentration (MIC) results from a study on Enterococcus faecium. Susu Chen published this data on figshare in April 2026.

Use Cases

• Analyze enzyme kinetic parameters like Michaelis constant (Km) and kcat/Km for ANT(9)-If activity on spectinomycin.
• Compare minimum inhibitory concentration (MIC) fold-increases between control and recombinant bacterial strains.
• Study the distribution and sequence identity of ant(9)-If(-like) genes across plasmids and chromosomes in Gram-positive bacteria.

Strengths

• Includes specific enzyme kinetic parameters: Km of 4.599 ± 0.198 μM and kcat/Km of (8.78 ± 0.39) × 10^4 M^–1⋅s^–1.
• Reports a 32-fold increase in spectinomycin MIC for the recombinant strain containing ant(9)-If.

Limitations

• Dataset is very small at 11.2 KB, indicating limited scope and likely a single table of summary results.
• Contains no raw sequencing or experimental data, only characterized findings from one bacterial isolate.

Provenance

Source

figshare, authored by Susu Chen.

Collection Method

Data derived from molecular cloning, protein expression, enzyme kinetics analysis, and bioinformatic sequence examination of Enterococcus faecium isolate 520.

Time Range

null

Freshness

Last updated in April 2026.

Geography

Bacterial isolate sourced from animal farm sewage; specific location not stated.