Comparative Study of tNGS vs. Traditional Pathogen Detection Methods in LRTIs, 2023–2025

562 patients with suspected lower respiratory infections from 29 departments were enrolled in a retrospective study during 2023–2025. Bronchoalveolar lavage fluid samples underwent parallel testing with targeted next-generation sequencing (tNGS) and conventional microbiological tests (CMTs). The dataset, authored by Zhengyuan An and last updated in May 2026, compares detection rates, turnaround times, and clinical outcomes.

Use Cases

• Compare pathogen detection rates between tNGS and conventional methods based on the reported primary outcomes.
• Analyze the impact of tNGS-guided therapy on hospital stay length and mortality based on the adjusted regression results.
• Investigate antibiotic modification rates following tNGS adoption based on the secondary outcome described.
• Assess the sensitivity of different diagnostic methods for viruses, fungi, and special pathogens based on the comparative results.

Strengths

• Includes data from 562 patients, providing a substantial clinical cohort.
• Reports specific, statistically significant results such as an 89.5% pathogen detection rate for tNGS versus 62.1% for CMTs.
• Covers multiple clinical outcomes including antibiotic modification rates, length of stay, and 28-day mortality.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect temporal and institutional bias inherent to the single retrospective study design.

Provenance

Source

figshare, authored by Zhengyuan An.

Collection Method

Retrospective study comparing diagnostic methods on patient samples.

Time Range

2023–2025

Freshness

Last updated 2026-05-08 05:52:33; freshness should be verified.

Geography

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