ANT(9)-Ie: Novel Spectinomycin Resistance Gene Characterization in Providencia

A 9.3 KB Excel file from figshare details the discovery of a novel chromosomally encoded aminoglycoside resistance gene, ant(9)-Ie. The dataset, authored by Lulu Huang and last updated in June 2026, presents results from a study on Providencia rettgeri P17 isolated from animal farm wastewater. It includes findings on the gene's function, kinetic parameters, and comparative genomic analysis.

Use Cases

• Study the genetic basis of spectinomycin resistance based on the characterization of the ant(9)-Ie gene.
• Compare amino acid sequence similarity of resistance proteins based on the reported 58.67% similarity to ANT(9)-Id.
• Analyze the genetic environment of resistance genes in Providencia strains based on the described conserved genetic context.
• Model enzyme kinetics for aminoglycoside-modifying enzymes based on the reported catalytic efficiency (kcat/Km) for spectinomycin.

Strengths

• Includes specific kinetic parameter for the ANT(9)-Ie enzyme: (8.22 ± 1.24) × 10^2 M−1·s−1.
• Reports a 64-fold increase in the minimum inhibitory concentration (MIC) of spectinomycin for the recombinant strain.
• Data is associated with a specific, peer-reviewed research methodology described in the background.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 9.3 KB file size suggests a very limited scope, likely containing summary results rather than raw experimental data.

Provenance

Source

figshare, author Lulu Huang.

Collection Method

Data derived from bacterial isolation, molecular cloning, heterologous expression, kinetic analysis, whole-genome sequencing, and comparative genomics as described.

Freshness

Last updated 2026-06-02 04:28:48; freshness should be verified.

Geography

Source bacterium isolated from wastewater from an animal farm; specific location not stated.