NtDof Gene Family in Tobacco: 75 Members with Evolutionary and Expression Analysis

A genome-wide identification and analysis of 75 DNA-binding with One Finger (Dof) transcription factor genes in tobacco (Nicotiana tabacum L.). The dataset, created by Bolun Zhi and last updated on 2026-04-28, includes phylogenetic classification, gene structure analysis, cis-regulatory element prediction, and expression profiling under cold and magnesium deficiency stresses.

Use Cases

• Phylogenetic classification of Dof transcription factors based on the described seven subfamilies.
• Analysis of gene structure and motif composition, particularly for the described CDF clade.
• Identification of stress- and hormone-responsive cis-regulatory elements from the predicted elements.
• Comparative expression profiling of NtCDF genes under cold and magnesium deficiency conditions.
• Functional validation studies on gene roles in stress tolerance, referencing the described VIGS experiments on NtDof40 and NtDof63.

Strengths

• Contains data on 75 identified NtDof gene members.
• Includes multiple analysis layers: phylogenetic reconstruction, gene structure, motif composition, and cis-element prediction.
• Expression profiling data for genes under specific abiotic stresses (cold and magnesium deficiency).
• Functional validation data from virus-induced gene silencing (VIGS) experiments on two representative genes.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (9.5 KB), indicating limited scope or highly summarized data.

Provenance

Source

figshare

Collection Method

Genome-wide bioinformatics identification and analysis, supplemented by experimental validation (VIGS).

Time Range

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Freshness

Last updated 2026-04-28 05:28:11; freshness should be verified.

Geography

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