Flow Cytometry Evaluation of Six Antimicrobial Agents Against Bacterial Isolates

165 antibiotic-isolate combinations were tested to compare flow cytometry with conventional broth microdilution for antimicrobial susceptibility testing. Essential agreement between the two methods was 90.71%, with categorical agreement reaching 100% for meropenem-vaborbactam. The dataset, authored by Emily Salisbury and last updated in May 2026, demonstrates flow cytometry's potential for rapid novel antimicrobial evaluation.

Use Cases

• Benchmarking rapid diagnostic methods based on the comparison of flow cytometry to broth microdilution.
• Analyzing cellular-level drug effects based on the observation of cell elongation under cefiderocol exposure.
• Evaluating categorical agreement for susceptibility testing based on EUCAST and CLSI breakpoints provided for three antibiotics.

Strengths

• Provides performance metrics for 165 distinct antibiotic-isolate combinations.
• Reports specific agreement percentages: 90.71% essential agreement and up to 100% categorical agreement.
• Includes results for six specific antimicrobial agents, including novel and niche drugs.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 15.2 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Experimental laboratory study comparing flow cytometry to broth microdilution.

Time Range

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Freshness

Last updated 2026-05-04 05:30:15; freshness should be verified.

Geography

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