High-Throughput miRNA Screen Against Intracellular Staphylococcus aureus

Figshare hosts a research document detailing a high-throughput screen of 2,469 human miRNA mimics in A549 epithelial cells infected with S. aureus USA300. The study identified miR-4430, miR-147a, and miR-1249-5p as multifunctional antimicrobial candidates and includes RNA-seq profiling data on host responses. The author is Pablo Castañera, and the dataset was last updated in March 2026.

Use Cases

• Analyze the high-throughput screening results for the 2,469 miRNA mimics to identify patterns in host-directed antimicrobial activity.
• Validate the prioritized candidate miRNAs (miR-4430, miR-147a, miR-1249-5p) across different S. aureus strains and epithelial cell lines as described in the methods.
• Examine the transcriptomic RNA-seq profiling data to study host responses, including STAT1- and PTAFR-associated signaling programs modulated by miR-4430.
• Investigate the complementary pathway-level programs, such as extracellular matrix organisation and integrin-mediated adhesion, associated with miR-1249-5p and miR-147a.

Strengths

• The dataset is based on a high-throughput screen of 2,469 human miRNA mimics, providing a broad initial candidate pool.
• Includes validation results across different S. aureus strains and epithelial cell lines, enhancing biological relevance.
• Contains transcriptomic RNA-seq profiling data linking miRNA candidates to specific host response pathways.

Limitations

• The primary data is a 659.7 KB DOCX file, which is a small document format not structured for direct computational analysis.
• No structured tabular data (rows, columns) or sample data is provided, limiting immediate use for quantitative modeling.
• The dataset's scope is limited to the specific experimental context of A549 cells and S. aureus USA300 infection.

Provenance

Source

Figshare, authored by Pablo Castañera.

Collection Method

High-throughput screen of miRNA mimics in infected epithelial cells, followed by network centrality analysis and RNA-seq profiling.

Freshness

Last updated March 2026.