In Vitro Generation of RORγt+ Regulatory T Cells: Protocol and Functional Data

A protocol and associated data for generating RORγt+ regulatory T cells in vitro, achieving up to a 70% frequency increase. The dataset, authored by Marcella Cipelli and last updated on 2026-05-21, enables functional and metabolic studies of this immune cell subset.

Use Cases

• Optimizing in vitro T cell differentiation protocols based on the described methodology.
• Comparing immunosuppressive function between RORγt+ Tregs and conventional induced Tregs.
• Investigating metabolic dependencies, such as oxidative phosphorylation, in specialized T cell subsets.

Strengths

• The protocol increased the frequency of target RORγt+ Treg cells by up to 70%.
• The dataset is openly available under a CC-BY-4.0 license.
• The description provides specific functional outcomes, such as enhanced immunosuppressive activity.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (14.7 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

In vitro cell differentiation and functional/metabolic assays.

Freshness

Last updated 2026-05-21 05:35:02