Mouse Duodenal Transcriptome and Microbiome Data for Ochratoxin A-Induced Ferroptosis

A seven-day mouse study exposing subjects to Ochratoxin A (OTA) at 2 mg/kg body weight/day. The dataset contains results from transcriptomic analysis (769 differentially expressed genes), 16S rRNA microbiome sequencing, and biochemical assays, authored by Shaokat Ali and last updated in April 2026. It links mycotoxin exposure to duodenal damage via ferroptosis and microbial dysbiosis.

Use Cases

• Identify ferroptosis-related gene expression patterns based on the 769 differentially expressed genes (DEGs) and 26 overlapping ferroptosis-regulating genes.
• Analyze shifts in gut microbial communities based on 16S rRNA sequencing data showing altered Bacteroidota abundance and pathogenic enrichment.
• Model the relationship between biochemical markers (MDA, iron, glutathione) and histological damage (villus atrophy, crypt hyperplasia) described in the study.
• Validate key ferroptosis regulator genes (SLC7A11, GSTM1, CP, SLC40A1) in the context of redox and iron homeostasis pathways.

Strengths

• Includes multi-modal experimental data: transcriptomics (769 DEGs), microbiome profiling (16S rRNA), and biochemical assays (MDA, iron, glutathione).
• Study design is clearly defined: 2 mg/kg/day OTA exposure for seven days in mice with a control group.
• Released under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (18.6 KB), suggesting it may contain summary results rather than raw sequencing or full assay data.

Provenance

Source

figshare, author Shaokat Ali.

Collection Method

Data generated from a controlled mouse study using histological analysis, TEM, transcriptomics, qRT-PCR, Western blot, immunofluorescence, biochemical assays, and 16S rRNA sequencing.

Time Range

Covers a seven-day experimental period.

Freshness

Last updated 2026-04-13 04:35:22; freshness should be verified.

Geography

Laboratory study; geographic origin of subjects is unspecified.