Melanoma Stem Cell and Macrophage Interaction Data

Two melanoma cell lines, A375 and WM115, were used to model stemness. Stem cell-conditioned media promoted THP-1 monocyte migration and induced a non-canonical macrophage phenotype, which was associated with shorter overall survival in an independent patient cohort. The dataset supports analysis of a bidirectional melanoma stem cell-macrophage axis and its effects on NK-cell function.

Use Cases

• Analyzing monocyte migration in response to stem cell signals based on CCR2 inhibition experiments.
• Profiling macrophage transcriptomic programs induced by melanoma stem cell-conditioned media.
• Investigating the impact of educated macrophages on NK-cell cytotoxicity.
• Correlating stem cell-educated macrophage gene signatures with patient survival outcomes.
• Studying cell line-dependent changes in stemness-associated transcriptional regulators.

Strengths

• Includes RNA-seq transcriptomic data from macrophage profiling.
• Links in vitro findings to clinical relevance via an independent melanoma patient cohort.
• Uses two established melanoma cell line models (A375 and WM115).

Limitations

• Specific column names and row counts are not provided in the metadata.
• Data is derived from in vitro models, which may not fully recapitulate physiological systems.
• The dataset consists of multiple file formats (XLSX, DOCX) with unclear structural relationships.

Provenance

Source

Martina Anselmi

Collection Method

Experimental data from melanosphere cultures, THP-1 monocyte differentiation, RNA-seq, and analysis of a clinical cohort.

Freshness

Last updated on 2026-06-02.