Optimized Lipid Extraction Protocol for Individual Mesozooplankton

Jiwoon Hwang's 2026 figshare publication details a methodological workflow for lipidomics on individual chitinous mesozooplankton. The protocol achieves a 2.5-fold increase in lipid recovery and a 4.4-fold gain in signal intensity using an optimized Bligh and Dyer extraction with in-line glass bead homogenization. It compares LOBSTAHS and MS-DIAL annotation pipelines and integrates a wax ester-specific fragmentation library.

Use Cases

• Compare LOBSTAHS and MS-DIAL annotation pipelines to assess systematic biases in lipidomic profiles.
• Apply the optimized Bligh and Dyer extraction protocol for high-resolution lipidomics on single Calanus copepods.
• Utilize the integrated wax ester-specific fragmentation library to improve annotation of marine-relevant lipid classes.

Strengths

• Method validated with a 2.5-fold increase in lipid recovery and a 4.4-fold gain in signal intensity.
• Workflow enables robust detection of intact lipid species from single Calanus copepods without specialized equipment.
• Publication is recent, last updated in March 2026.

Limitations

• Dataset is a 101.3 KB PDF document, not a structured data table, limiting direct computational analysis.
• No tabular data, rows, or columns are provided; the resource is a methodological description.
• The specific sample size or number of individual zooplankton analyzed is not stated.

Provenance

Source

Jiwoon Hwang via figshare

Collection Method

Developed and validated laboratory protocol for lipid extraction and annotation from individual mesozooplankton.

Freshness

2026-03-23