Table 8: Antibody CDR3-VH Signatures for Oligonucleotide Recognition

Riccardo Galasso's dataset provides a detailed analysis of antibody repertoire remodeling during phage display selection against phosphorothioate-modified antisense oligonucleotides (PS-ASOs). It contains a shared enriched set of 113 CDR3-VH clonotypes, characterized by physicochemical properties like increased positive charge and constrained length distribution. The data supports the identification of reproducible biophysical signatures associated with PS-ASO recognition.

Use Cases

• Identifying shared CDR3-VH clonotypes enriched during phage display selection against PS-ASOs.
• Analyzing physicochemical profiles, such as charge and hydrophobicity, of antibody CDR3 regions.
• Validating antibody binding specificity through comparison with phosphodiester-backbone oligonucleotide controls.
• Correlating next-generation sequencing enrichment data with functional ELISA and microscopy assays.

Strengths

• Contains a specifically identified set of 113 CDR3-VH clonotypes.
• Data is derived from two independent, two-round phage display biopanning strategies coupled with Illumina MiSeq sequencing.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Column names and a precise row count are not provided in the metadata, limiting immediate understanding of the data structure.
• The dataset appears as multiple separate uploads on the same platform with slightly different file sizes, suggesting potential versioning or upload inconsistencies.

Provenance

Source

Riccardo Galasso

Collection Method

Generated from next-generation sequencing-guided phage display experiments and subsequent bioinformatic analysis.

Freshness

Last updated on 2026-05-29.