Table 6: Antibody CDR3-VH Repertoires Recognizing Phosphorothioate Oligonucleotides

Riccardo Galasso's dataset contains sequencing and analysis results from a phage display campaign targeting phosphorothioate-modified antisense oligonucleotides. It includes a shared enriched set of 113 CDR3-VH clonotypes, characterized by physicochemical profiles such as charge, aromatic residue patterns, and hydrophobicity. The data supports the identification of antibody signatures for binding to modified therapeutic oligonucleotides.

Use Cases

• Identifying shared antibody CDR3 sequence motifs based on next-generation sequencing data.
• Analyzing physicochemical convergence (e.g., charge, pI, hydrophobicity) in antibody repertoires.
• Validating phage display selection outcomes via ELISA binding and competition assays.
• Correlating CDR3 sequence features with specificity for phosphorothioate versus phosphodiester oligonucleotide backbones.

Strengths

• Dataset is derived from two independent phage display biopanning strategies, suggesting reproducibility.
• Analysis identifies a specific set of 113 enriched CDR3-VH clonotypes with defined physicochemical signatures.
• Data is licensed under CC-BY-4.0, permitting open reuse.

Limitations

• Column names and exact row counts are not provided in the metadata, limiting immediate understanding of data structure.
• Multiple entries on the same platform show slight discrepancies in reported file sizes (11339, 12439, 13679).
• The dataset appears to be a single analysis table; its scope is confined to the described experimental campaign.

Provenance

Source

Riccardo Galasso.

Collection Method

Data generated from next-generation sequencing (Illumina MiSeq) of phage display libraries, processed through a bioinformatic pipeline for clonotype reconstruction and analysis.

Freshness

Last updated 2026-05-29.