Pyrazinamide Resistance Detection Data: Broth Microdilution and PCR Assay Validation

A dataset comparing methods for detecting pyrazinamide resistance in rifampicin-resistant tuberculosis isolates. The study evaluated broth microdilution at two critical concentrations and a fluorescence PCR melting curve assay against whole-genome sequencing. Yanfeng Zhao published the data on figshare in May 2026.

Use Cases

• Compare diagnostic agreement between phenotypic and genotypic resistance testing based on the described BMD and MeltPro assay results.
• Evaluate critical concentration thresholds for drug susceptibility testing based on the 100 μg/mL and 200 μg/mL BMD resistance rates.
• Validate rapid molecular assays for pncA mutation detection based on the concordance metrics with whole-genome sequencing.
• Assess the performance of fluorescence PCR melting curve analysis for tuberculosis isolates based on the reported 89.7% agreement for mutation-positive samples.

Strengths

• Includes 110 rifampicin-resistant tuberculosis isolates, providing a defined sample size.
• Reports specific agreement statistics (e.g., κ=0.872 for BMD at 200 μg/mL) with whole-genome sequencing.
• Compares two critical concentrations (100 μg/mL and 200 μg/mL) for the broth microdilution method.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to the collection from Beijing institutions.

Provenance

Source

figshare

Collection Method

Broth microdilution and fluorescence PCR melting curve analysis performed on clinical isolates, with whole-genome sequencing as a comparator.

Freshness

Last updated 2026-05-25 05:31:07; freshness should be verified.

Geography

Isolates were collected from tuberculosis prevention and control institutions and designated hospitals in Beijing.