Transcriptional Profiles of Pseudomonas aeruginosa Biofilm Life Cycle Stages

A dataset from figshare containing transcriptional profiling data for the model bacterium Pseudomonas aeruginosa PAO1. The work characterizes gene expression across three biofilm life cycle stages—attachment, maturation, and dispersal—in closed cultures. The dataset, authored by Xavier Bertran Forga and last updated in April 2026, identifies stage-specific gene upregulation and proposes fourteen genes as transcriptional biomarkers of dispersal.

Use Cases

• Identify gene expression biomarkers for biofilm dispersal based on reported upregulation of genes like amrZ, dspI, and nbdA.
• Analyze stage-specific adaptations in bacterial biofilms based on transcriptional profiles for attachment, maturation, and dispersal.
• Validate reporter plasmid tools for detecting biofilm dispersal onset using the identified subset of fourteen genes.
• Study the role of c-di-GMP-related enzymes and phosphodiesterases in biofilm regulation based on reported fold-change data.

Strengths

• Provides specific fold-change data for gene upregulation across biofilm stages, such as a 6.27-fold increase for amrZ during dispersal.
• Benchmarks stage-specific transcriptional profiles for a model biofilm-forming bacterium (Pseudomonas aeruginosa PAO1).
• Identifies a concrete subset of fourteen genes proposed as transcriptional biomarkers of dispersal.
• Dataset is openly licensed under CC-BY-4.0.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is contained in a DOCX file (1.9 MB), which may require extraction or conversion for computational analysis.

Provenance

Source

figshare, author Xavier Bertran Forga.

Collection Method

Transcriptional profiling of Pseudomonas aeruginosa PAO1 biofilm stages in closed cultures.

Time Range

Experimental timeframe not specified in metadata.

Freshness

Last updated 2026-04-16 05:25:27; freshness should be verified.

Geography

Geographic scope not specified in metadata.