Table 2_Transcriptomic profiling reveals substrate- and shear stress-dependent maturation

Bulk RNA sequencing experiments investigated the response of human intestinal stem cell-derived monolayers to biomimetic substrates and shear stress. The dataset, authored by Nadra Alzain and last updated in May 2026, likely contains gene expression data from cells cultured on decellularized small intestinal submucosa (dSIS-MA) hydrogels versus Matrigel-coated plastic under static and dynamic fluid flow conditions. Longitudinal analysis revealed transcriptional remodeling toward metabolic, immune, and absorptive lineage pathways.

Use Cases

• Compare gene expression signatures between dSIS-MA hydrogel and Matrigel-coated plastic substrates based on the described experimental setup.
• Analyze transcriptional adaptation to shear stress in dynamic culture conditions based on the described millifluidic system.
• Identify genes associated with absorptive lineage maturation and reduced crypt-associated signatures based on the reported findings.
• Study the activation of metabolic and immune pathways under controlled fluid flow based on the description of transcriptional remodeling.

Strengths

• Dataset is derived from primary human intestinal epithelial cells, a biologically relevant source.
• Experiments include longitudinal analysis, suggesting time-series data on gene expression stability.
• The study compares multiple conditions: two substrate types and static vs. dynamic culture.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small in scale at 131.6 KB, indicating limited data volume.

Provenance

Source

Nadra Alzain

Collection Method

Bulk RNA sequencing of human intestinal stem cell-derived monolayers cultured on custom hydrogels in a millifluidic system.

Freshness

Last updated 2026-05-01 04:16:28