Virus-Induced Gene Editing in Groundcherry: Somatic and Heritable Mutations

This dataset documents a study establishing Tobacco rattle virus-mediated gene editing in groundcherry (Physalis grisea). It reports somatic editing frequencies of 80–95% for the PDS gene and up to 73% for CLV1, with heritable edits recovered in progeny. The study involved Cas9-expressing plants, with all five PDS-targeted plants producing edited offspring.

Use Cases

• Analyze somatic editing frequencies for the PDS and CLV1 genes to compare VIGE efficiency across targets.
• Study the heritability of edits by examining progeny data from T0 plants, including fully albino seedlings with frameshift mutations.
• Investigate phenotypic outcomes like increased floral organ number and multilocular fruits in CLV1-edited plants.
• Examine editing patterns in a tetraploid context, including mono-, bi-, tri-, and tetra-allelic mutations generated.

Strengths

• Reports high somatic editing frequencies (80–95% for PDS, up to 73% for CLV1).
• Demonstrates heritable edits, with 100% of PDS-targeted plants (n=5) and 60% of CLV1-targeted plants producing edited progeny.
• Documents editing outcomes in a tetraploid plant, showing robust mutation across four alleles.

Limitations

• The dataset is a single 32.3 KB PDF, containing summarized results rather than raw experimental data or tabular records.
• Sample sizes are small (e.g., n=5 for PDS), limiting statistical power for broader inferences.
• The primary Cas9-expressing line was tetraploid due to an artifact of tissue culture, which may not represent typical diploid editing scenarios.

Provenance

Source

figshare

Collection Method

Experimental study using Tobacco rattle virus (TRV) to deliver guide RNAs into Cas9-expressing groundcherry plants.

Freshness

Last updated March 20, 2026.