Bovine Sperm Proteomics Before and After Cryopreservation, 1,373 Proteins

A 2026 proteomic analysis by Alexandra Keller compares protein abundance in bovine sperm before and after cryopreservation. The dataset contains results from five bulls, identifying 1,373 proteins and 84 with significant differential abundance. The work was published on figshare under a CC-BY-4.0 license.

Use Cases

• Train classification models to predict cryo-damage susceptibility based on the 84 differentially abundant proteins.
• Analyze protein-protein interaction networks based on functional enrichment results for metabolic and reproductive processes.
• Validate biomarker candidates like CYCT, DDOST, and RPS3A using the provided proteomic profiles.
• Study pathways related to mitochondrial integrity and cytoskeletal organization disrupted during cryopreservation.

Strengths

• Includes 1,373 identified proteins after filtering, providing a substantial proteomic profile.
• Quantifies 84 proteins with significant differential abundance (log2FC > 2, p < 0.05).
• Data is derived from five sexually mature bulls with proven fertility, suggesting biological relevance.
• Results are supported by functional enrichment analysis and image-based flow cytometry validation.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (109.6 KB), indicating limited scope, likely containing summary results rather than raw spectra.

Provenance

Source

figshare, author Alexandra Keller.

Collection Method

Bottom-up proteomic approach using LC-MS/MS on semen samples from five bulls, divided into fresh and cryopreserved groups.

Time Range

Study likely conducted prior to 2026 publication date; specific collection dates are not provided.

Freshness

Last updated 2026-04-16 05:26:14; freshness should be verified.

Geography

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