Comparative Study of tNGS vs. Traditional Pathogen Detection in LRTIs, 2023–2025

A 2023–2025 retrospective study of 562 patients with suspected lower respiratory tract infections, comparing targeted next-generation sequencing (tNGS) to conventional microbiological tests. The dataset, authored by Zhengyuan An and shared on figshare, reports pathogen detection rates, turnaround times, and clinical outcomes including antibiotic modifications, length of stay, and mortality.

Use Cases

• Benchmarking diagnostic sensitivity and specificity based on reported pathogen detection rates for tNGS and CMTs.
• Modeling the impact of diagnostic-guided therapy on patient outcomes like hospital stay length and mortality based on the study's adjusted analysis.
• Analyzing antibiotic modification rates following tNGS results as described in the patient management cohort.
• Comparing detection performance for specific pathogen categories (viruses, fungi, special pathogens) between the two methods.

Strengths

• Includes data from 562 patients across 29 departments, providing a multi-center clinical perspective.
• Reports specific, statistically significant performance metrics, such as an 89.5% pathogen detection rate for tNGS versus 62.1% for CMTs.
• Contains outcome measures adjusted via multivariable logistic regression and propensity score analysis, suggesting methodological rigor.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 134.0 KB file size indicates a limited scope, likely containing summary or aggregated results rather than raw patient-level data.

Provenance

Source

Zhengyuan An via figshare

Collection Method

Retrospective study with parallel testing of bronchoalveolar lavage fluid samples using tNGS and conventional methods.

Time Range

2023–2025

Freshness

Last updated 2026-05-08 05:52:33; freshness should be verified.

Geography

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