ANT(9)-Ie Resistance Gene Characterization in Providencia Rettgeri P17

Lulu Huang published a dataset on 2026-06-02 detailing the discovery and characterization of a novel spectinomycin resistance gene, ant(9)-Ie, in Providencia rettgeri P17. The data includes results from minimum inhibitory concentration (MIC) tests, kinetic analysis of the ANT(9)-Ie enzyme, and comparative genomic analysis of the gene's environment. The dataset is 17.6 KB in size and is available in XLSX format under a CC-BY-4.0 license.

Use Cases

• Training models to predict antimicrobial resistance based on genetic features mentioned in the description
• Comparative genomic analysis of resistance gene environments based on the conserved genetic context described
• Studying enzyme kinetics and catalytic efficiency for aminoglycoside-modifying enzymes based on the reported kcat/Km values
• Analyzing the distribution and evolution of ant(9) gene variants based on the phylogenetic analysis referenced

Strengths

• Dataset includes specific kinetic parameters for the ANT(9)-Ie enzyme, such as a spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Results include a quantified 64-fold increase in the minimum inhibitory concentration (MIC) of spectinomycin for the recombinant strain.
• Analysis is based on whole-genome sequencing and comparative genomics, as stated in the methods.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's 17.6 KB size suggests a limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare, author Lulu Huang.

Collection Method

Data generated from bacterial isolation from animal farm wastewater, MIC determination, molecular cloning, heterologous expression, kinetic analysis, and whole-genome sequencing.

Freshness

Last updated 2026-06-02 04:28:47; freshness should be verified.