Evolutionary Analysis of the Dof Gene Family in Tobacco with 75 Members

A genome-wide identification and analysis of 75 Dof transcription factor genes in tobacco (Nicotiana tabacum L.). The dataset includes phylogenetic classification, gene structure analysis, cis-regulatory element prediction, and expression profiling under cold and magnesium deficiency stress. It was authored by Bolun Zhi and last updated on 2026-04 28.

Use Cases

• Classify Dof transcription factors into subfamilies based on phylogenetic reconstruction mentioned in the description
• Analyze gene structure and motif composition for evolutionary insights
• Identify stress- and hormone-responsive cis-regulatory elements in promoter regions
• Study gene expression dynamics in response to cold and magnesium deficiency stresses
• Investigate functional roles of specific genes (e.g., NtDof40, NtDof63) in cold tolerance using VIGS data

Strengths

• Identifies 75 specific Dof gene members in tobacco, providing a concrete count
• Includes multiple analysis layers: phylogenetics, gene structure, cis-elements, and expression profiling
• Functional validation data from Virus-Induced Gene Silencing (VIGS) experiments for two genes

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download
• The dataset is very small (14.4 KB), indicating limited scope, likely containing summary results rather than raw sequencing data

Provenance

Source

figshare

Collection Method

Genome-wide bioinformatics analysis and experimental validation (VIGS) as described.

Time Range

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Freshness

Last updated 2026-04-28 05:28:01; freshness should be verified

Geography

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