Sisal SBP Gene Family: 45 Identified Genes with Expression Under Pathogen Stress

Loaded with genome-wide identification of 45 AhSBP genes in Agave sisalana, with 18 identified as putative miR156 targets. It integrates comparative transcriptomics, promoter cis-element analysis, and RT-qPCR validation to elucidate the miR156-AhSBP module's role under pathogen stress. The analysis reveals lineage-specific expansion driven by segmental duplication (70%) and differential expression in susceptible versus resistant cultivars.

Use Cases

• Analyze the expression patterns of 45 AhSBP genes, such as AhSBP9 and AhSBP38, under pathogen stress conditions.
• Identify putative miR156 target genes among the 18 AhSBP candidates to study post-transcriptional regulation.
• Compare transcriptomic data between susceptible and resistant cultivars to correlate asi-miR156 induction levels with gene suppression.
• Examine promoter cis-elements of AhSBP genes to infer regulatory mechanisms involved in plant defense.
• Investigate the functional roles of specific downstream effectors like AhSBP17 and AhSBP34 in sisal immune homeostasis.

Strengths

• Identifies 45 AhSBP genes from a genome-wide analysis, providing a specific gene family count.
• Integrates multiple analysis methods: phylogenetic modeling, synteny, transcriptomics, and RT-qPCR validation.
• Findings are based on comparative analysis between susceptible and resistant cultivars, offering a contrast.

Limitations

• The dataset is small at 12.8 KB, indicating limited scope and likely summary-level data rather than raw observations.
• Sample data and column structure are unavailable, limiting immediate analytical utility without further investigation.
• Focus is on a single plant species (Agave sisalana), reducing generalizability to other organisms.

Provenance

Source

figshare, authored by Zhiwei Lu.

Collection Method

Genome-wide identification, phylogenetic and synteny modeling, comparative transcriptomics, promoter cis-element analysis, and RT-qPCR validation.

Freshness

Last updated 2026-03-20.