Genomic Determinants of Antifungal Activity in Streptomyces Strain STM-2

This dataset details the genomic analysis of Streptomyces melanosporofaciens strain STM-2, isolated from Chiayi County, Taiwan. It includes predictions of 52 biosynthetic gene clusters (BGCs) and identifies genes for antifungal enzymes like chitinases and proteases.

Use Cases

• Identify and analyze the 52 predicted biosynthetic gene clusters (BGCs) for type I/II PKS, NRPS, and hybrid systems.
• Examine genes for antifungal hydrolytic enzymes, including chitinases, β-1,3-glucanases, and proteases, to understand biocontrol mechanisms.
• Use the high-GC (71%) linear chromosome sequence for comparative pangenomic analysis with other Streptomyces strains.
• Investigate the accessory genome and unique genes associated with specialized antifungal activity through evolutionary analysis.

Strengths

• Genome mining predicted an extensive repertoire of 52 biosynthetic gene clusters (BGCs).
• The whole-genome sequence has a high-GC content of 71%.
• Comparative analysis includes high-resolution Average Nucleotide Identity (ANI) and digital DNA–DNA hybridization (dDDH) scores.

Limitations

• The dataset is very small at 12.1 KB, indicating limited scope, likely a summary table rather than raw genomic data.
• Rows and columns are unknown, limiting assessment of data volume and feature completeness.
• Data is derived from a single novel strain (STM-2), limiting generalizability to other organisms.

Provenance

Source

Author: Muhammed Opoku Gyamfi via figshare.

Collection Method

Integrated approach involving physiological/biochemical characterization, genome sequencing, annotation, mining with antiSMASH, comparative genomics, and in silico structural analysis with AlphaFold2.

Freshness

Last updated on 2026-03-20.

Geography

Streptomyces melanosporofaciens strain STM-2 was isolated from Chiayi County, Taiwan.