Pinellia Ternata Alkaloid Biosynthesis: Key Genes and Functional Verification

63,106 unigenes from transcriptomic profiling of two Pinellia ternata varieties with distinct alkaloid profiles. The dataset, created by Yanyan Pei and last updated in 2026, likely contains results from LC-MS analysis, transcriptome sequencing, and gene overexpression experiments in callus tissue. It provides a molecular framework for understanding alkaloid synthesis regulation in this medicinal plant.

Use Cases

• Identify differentially expressed genes in alkaloid pathways based on transcriptomic profiling of two plant varieties.
• Validate gene function in alkaloid metabolism based on results from overexpression experiments in callus tissue.
• Map putative biosynthetic pathways for monoterpenoid indole and benzylisoquinoline alkaloids based on annotated unigenes.
• Screen for key enzyme and transcription factor genes involved in secondary metabolism based on reference sequence comparisons.

Strengths

• Includes results from functional verification via gene overexpression experiments, providing direct evidence for gene roles.
• Transcriptomic profiling yielded 63,106 annotated unigenes, offering a substantial genetic resource.
• Compares two cultivated varieties with distinct alkaloid profiles, enabling differential analysis.

Limitations

• Row count and specific column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small (67.4 KB), indicating limited scope, likely containing summary results rather than raw sequencing data.
• Last updated 2026-04-28 05:28:20; freshness should be verified as it is a future date.

Provenance

Source

figshare, author Yanyan Pei

Collection Method

LC-MS analysis, transcriptome sequencing, and gene overexpression in Pinellia ternata callus.

Freshness

Last updated 2026-04-28 05:28:20