In Vitro Generation of RORγt+ Regulatory T Cells: Protocol and Functional Data

An optimized protocol increased the frequency of RORγt+ Treg cells in vitro by up to 70%. The dataset, authored by Marcella Cipelli and shared on figshare, includes results on the immunosuppressive function and metabolic profile of these cells. It was last updated on 2026-05-21.

Use Cases

• Benchmarking T cell differentiation protocols based on the described in vitro generation method.
• Studying immunosuppressive function based on the comparison to conventional induced Treg cells.
• Analyzing metabolic dependencies in immune cells based on the reported reliance on oxidative phosphorylation (OXPHOS).

Strengths

• The protocol achieved a reported 70% increase in target cell frequency.
• Includes functional and metabolic analyses as described in the summary.
• Shared under a permissive CC-BY-4.0 license.

Limitations

• Dataset is very small at 14.4 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

In vitro cell differentiation and analysis as described in the protocol.

Freshness

Last updated 2026-05-21 05:35:02