Mouse Duodenal Transcriptome and Microbiome Data for Ochratoxin A Ferroptosis Study

Integrative transcriptomic and microbiome data from a mouse study investigating duodenal damage induced by Ochratoxin A (OTA). The dataset includes results from a 7-day exposure experiment with 2 mg/kg body weight/day OTA, revealing 769 differentially expressed genes and altered cecal microbiota profiles. Author Shaokat Ali published the data on figshare under a CC-BY-4.0 license, last updated in April 2026.

Use Cases

• Identify ferroptosis-related gene expression changes based on the 769 differentially expressed genes (DEGs) mentioned.
• Analyze links between pathogenic microbiota enrichment and duodenal damage based on 16S rRNA sequencing results.
• Model the impact of redox and iron homeostasis disruption using data on downregulated key genes (SLC7A11, GSTM1, CP, SLC40A1).
• Correlate histological and biochemical markers (e.g., MDA, iron, LPS levels) with transcriptomic profiles.

Strengths

• Integrates multiple data modalities: transcriptomics (769 DEGs), 16S rRNA microbiome sequencing, and biochemical assays.
• Experimental design is clearly described, including OTA dosage (2 mg/kg/day) and exposure duration (7 days).
• Specific ferroptosis hallmarks are documented, including mitochondrial shrinkage and altered antioxidant defenses (glutathione, SOD, CAT, T-AOC).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is from a single, controlled mouse study, limiting generalizability.

Provenance

Source

figshare, author Shaokat Ali.

Collection Method

Data generated from a mouse experiment using histological analysis, TEM, transcriptomics, qRT-PCR, Western blot, immunofluorescence, biochemical assays, and 16S rRNA sequencing.

Time Range

Experimental timeframe not specified; dataset reflects a 7-day exposure period.

Freshness

Last updated 2026-04-13 04:35:25; freshness should be verified.

Geography

Laboratory study; geographic origin of samples or institution is not specified.