Canine Visceral Hemangiosarcoma MicroRNA Differential Expression Analysis

A 2026 study by Laura Machado Ribas analyzed formalin-fixed, paraffin-embedded tissues from 24 dogs with histologically confirmed visceral hemangiosarcoma and 12 non-neoplastic controls. The dataset contains results from small RNA sequencing and quantitative PCR validation, identifying 67 and 71 differentially expressed miRNAs in splenic and cardiac tumors, respectively. Pathway enrichment analysis revealed significant involvement in oncogenic signaling pathways like PI3K-Akt and MAPK.

Use Cases

• Validate potential miRNA biomarkers for canine visceral hemangiosarcoma based on the 13 validated differentially expressed miRNAs.
• Conduct pathway enrichment analysis based on the oncogenic signaling pathways (PI3K-Akt, MAPK, HIF-1, Ras) identified in the study.
• Compare miRNA expression profiles between splenic and cardiac tumor subtypes based on the 18 shared differentially expressed miRNAs.
• Assess the utility of archived FFPE tissues for biomarker discovery as demonstrated in the study methodology.

Strengths

• Includes data from 36 samples (24 tumor, 12 control) providing a comparative baseline.
• Results are validated by RT-qPCR for 13 miRNAs, showing strong concordance with sequencing.
• Pathway analysis links findings to specific oncogenic signaling mechanisms.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (11.1 KB), indicating limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare, author Laura Machado Ribas.

Collection Method

Small RNA sequencing and quantitative PCR analysis of formalin-fixed, paraffin-embedded tissues.

Time Range

Study published in 2026.

Freshness

Last updated 2026-03-17 14:40:00; freshness should be verified.

Geography

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