Table 3_Optimized lipid extraction and annotation pipeline customization for individual ch

A methodological dataset from 2026 detailing an optimized lipid extraction and annotation pipeline for individual chitinous mesozooplankton. The data, authored by Jiwoon Hwang and shared under a CC-BY-4.0 license, supports high-resolution lipidomics to study trophic interactions and carbon cycling in marine ecosystems. The workflow achieved a 2.5-fold increase in lipid recovery and a 4.4-fold gain in signal intensity for single Calanus copepods.

Use Cases

• Benchmarking lipid extraction protocols based on the described 2.5-fold recovery increase and 4.4-fold signal gain.
• Comparing lipid annotation pipeline biases (e.g., LOBSTAHS vs. MS-DIAL) as discussed in the description.
• Improving annotation of marine-relevant lipid classes like wax esters using the described fragmentation library.
• Analyzing biological heterogeneity in lipid profiles from individual mesozooplankton specimens.
• Tracing lipid metabolism across trophic levels in marine ecosystems.

Strengths

• Method validated with specific quantitative gains: 2.5-fold lipid recovery increase and 4.4-fold signal intensity gain.
• Workflow designed for accessibility, requiring no additional homogenization equipment for single copepods.
• Includes comparison of computational annotation strategies (LOBSTAHS and MS-DIAL) to highlight systematic biases.
• Integrated a wax ester-specific fragmentation library to improve annotation for marine-relevant lipid classes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for statistical analysis.
• The dataset is very small (111.6 KB), indicating limited scope, likely containing methodological results rather than extensive sample data.

Provenance

Source

Jiwoon Hwang via figshare.

Collection Method

Optimized Bligh and Dyer–based lipid extraction with in-line glass bead homogenization, analyzed via UPLC-HRMS.

Time Range

Methodological data from 2026.

Freshness

Last updated 2026-03-23 06:30:54.

Geography

Marine ecosystems, with specific mention of Calanus copepods.