Pharmacological and Genetic Modulation of IL-32 Expression in Intestinal Epithelial Cells

Experimental data from a study investigating the role of IL-32 in HIV-1 latency and reactivation at the intestinal barrier. The dataset, shared by Etiene Moreira Gabriel on figshare under a CC-BY-4.0 license, contains results from cytokine treatments, CRISPR/Cas9 gene editing, and viral outgrowth assays. It was last updated on 2026-05-26.

Use Cases

• Analyze the effects of IL-22, ATRA, and RGZ on IL-32 isoform expression based on the cytokine treatment results.
• Compare transcriptional profiles between control and IL32-knockout intestinal epithelial cells based on the RNA-Sequencing analysis.
• Evaluate the impact of IL-32 modulation on HIV-1 outgrowth from CD4+ T-cell reservoirs based on the co-culture assay results.

Strengths

• Data is associated with a detailed, peer-reviewed-style methodology description.
• The dataset is shared under a permissive CC-BY-4.0 license for reuse.
• File size is 3.8 MB, indicating a focused and manageable dataset.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific cell lines and donor samples used.

Provenance

Source

figshare, author Etiene Moreira Gabriel

Collection Method

Laboratory experiments using HT-29 intestinal epithelial cells, CRISPR/Cas9 gene editing, RT-PCR/ELISA quantification, and viral outgrowth assays with CD4+ T-cells from ART-treated people with HIV.

Freshness

Last updated 2026-05-26 07:48:56; freshness should be verified.