Riccardo Galasso's dataset, last updated in 2026, contains physicochemical profiles of antibody complementarity-determining region 3 (CDR3-VH) sequences. It includes a shared enriched set of 113 CDR3-VH clonotypes identified from phage display selections against phosphorothioate-modified antisense oligonucleotides. The data was generated via Illumina MiSeq sequencing and bioinformatic analysis to study convergent binding signatures.
Use Cases
- Identify convergent physicochemical signatures in antibody repertoires based on the described CDR3-VH clonotype set.
- Prioritize candidate antibody binders for polyanionic targets based on features like positive charge and aromatic residue patterns.
- Study repertoire focusing and clonal dominance in phage display selections using the described NGS-guided framework.
Strengths
- Includes a defined set of 113 shared enriched CDR3-VH clonotypes.
- Physicochemical descriptors include positive charge, aromatic residue patterns, pI, and hydrophobicity.
- Data is associated with functional validation via ELISA and fluorescence microscopy assays mentioned in the description.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
- The dataset is very small at 11.5 KB, indicating limited scope.
Provenance
- Source
- figshare, author Riccardo Galasso.
- Collection Method
- Generated via next-generation sequencing-guided phage display and bioinformatic pipeline analysis.
- Freshness
- Last updated 2026-05-29 04:50:49; freshness should be verified.