Proteomic Analysis of Lysinibacillus PET Biodegradation Potential

18.3 KB proteomic dataset compares the early-stage growth of a PET-associated Lysinibacillus strain against the reference Ideonella sakaiensis over six weeks. The data includes proteomic analysis results, in silico screening for enzymes, and surface imaging observations from FE-SEM. It was authored by Radoslaw B. Dudziak and shared under a CC BY 4.0 license.

Use Cases

• Analyze proteomic data to identify oxidative-stress response proteins like peroxiredoxins and superoxide dismutases expressed under PET exposure.
• Compare early-stage growth assay results between Lysinibacillus sp. and Ideonella sakaiensis across multiple time points (Weeks 1, 2, 4, and 6).
• Use in silico screening results to examine the absence of canonical PET-degrading enzymes such as PETase or MHETase with the GXSXG motif.
• Correlate FE-SEM surface imaging observations of erosion, fissures, and delamination with proteomic metabolic reprogramming data.

Strengths

• Includes comparative growth assay data over a six-week experimental period.
• Proteomic analysis covers a range of protein types, including metabolic enzymes and oxidative-stress response proteins.
• Dataset is openly shared under a permissive CC BY 4.0 license.

Limitations

• The dataset is very small at 18.3 KB, indicating limited scope and likely a summary of results rather than raw experimental data.
• Proteomic analysis did not detect known PET-degrading enzymes, limiting direct applications for enzyme discovery.
• Specific row and column counts, sample sizes, and raw data details are unavailable.

Provenance

Source

Figshare, authored by Radoslaw B. Dudziak.

Collection Method

Data generated from growth assays, proteomics (MALDI-TOF MS), in silico screening, and surface imaging (FE-SEM) of a bacterial strain isolated from Icelandic soil.

Time Range

Experimental period covers six weeks.

Freshness

Last updated in March 2026.

Geography

Source bacterial strain isolated from Icelandic soil.