Mouse Wound Infection Data for EM86-Loaded Nanofibrous Dressing

Results from a study evaluating a novel antimicrobial peptide, EM86, loaded onto a gamma-irradiated sodium alginate/polyvinyl alcohol nanofibrous dressing for treating multidrug-resistant Pseudomonas aeruginosa wound infections in BALB/c mice. The dataset, created by Samar Essam Metwally, contains detailed experimental findings from in vitro and in vivo testing, including MIC/MBC values, time-kill kinetics, and wound bacterial counts. It was last updated on April 7, 2026, and is stored in a 26.2 KB DOCX file.

Use Cases

• Analyze the relationship between EM86 peptide loading (35 ± 18 μg) and the reduction in P. aeruginosa SM016 counts in mouse wounds after 4 days of treatment.
• Compare the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of EM86 against various MDR Gram-negative bacterial isolates.
• Examine time-kill kinetics data showing EM86 killed Pseudomonas aeruginosa SM016 within 60 minutes, with SEM-confirmed morphological changes after 30 minutes.
• Evaluate the safety profile of EM86 using its IC50 value (>300 μg/mL) from MTT assays on human skin fibroblasts (HSF).
• Correlate electrospinning parameters (30 kV, 0.045 mm/min) and polymer ratios (1:12.3 SA/PVA) with resulting nanofiber properties like mean diameter (238 ± 78 nm) and swelling capacity (510%).

Strengths

• Dataset provides specific, quantitative results including MIC/MBC values, IC50 > 300 μg/mL, and a 510% swelling capacity for the dressing.
• Includes in vivo efficacy data from BALB/c mice, showing significant bacterial count reduction (P < 0.001) after 4 days of treatment.
• Clear methodological provenance from a published study using computer-aided peptide design, SEM, and FTIR for validation.

Limitations

• Extremely small data volume (26.2 KB), indicating it is a summary document, not a raw experimental data repository.
• Sample size for the in vivo mouse model is not specified, limiting statistical power assessment.
• Data is presented in a narrative DOCX format, requiring manual extraction for computational analysis.

Provenance

Source

figshare, authored by Samar Essam Metwally.

Collection Method

Experimental data from synthesizing and testing a designed antimicrobial peptide (EM86) and its application in a nanofibrous wound dressing, including in vitro assays and an in vivo BALB/c mouse infection model.

Time Range

Study period not specified; dataset reflects a completed experiment.

Freshness

Last updated April 7, 2026.