Table 3_Toward precision detection of pyrazinamide resistance: critical concentration asse

A 2026 study by Yanfeng Zhao compared broth microdilution and a molecular assay for detecting pyrazinamide resistance in rifampicin-resistant tuberculosis isolates from Beijing. The dataset likely contains results from 110 isolates, with resistance rates of 63.6% and 45.5% at two critical concentrations. Agreement statistics with whole-genome sequencing genotypes are reported, including kappa values of 0.590, 0.872, and 0.882.

Use Cases

• Validate molecular diagnostic assays based on pncA mutation detection.
• Compare phenotypic and genotypic susceptibility testing methods for pyrazinamide.
• Assess critical concentration thresholds for broth microdilution tests in tuberculosis.

Strengths

• Provides specific performance metrics: resistance rates of 63.6% (70/110) and 45.5% (50/110).
• Reports agreement statistics (e.g., κ=0.872) with a genotypic comparator (whole-genome sequencing).
• Focuses on a clinically relevant population: rifampicin-resistant tuberculosis isolates.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data scope is limited to 671.8 KB, suggesting a focused, small-scale study.

Provenance

Source

Yanfeng Zhao via figshare

Collection Method

Broth microdilution and fluorescence PCR melting curve analysis performed on clinical isolates.

Freshness

Last updated 2026-05-25 05:31:07; freshness should be verified.

Geography

Isolates were collected from patients at TB prevention and control institutions and designated hospitals in Beijing.