Virus-Induced Gene Editing in Groundcherry: Somatic and Heritable Mutations

This dataset documents a study establishing Tobacco rattle virus-mediated gene editing in groundcherry (Physalis grisea). It reports somatic editing frequencies of 80–95% for the PDS gene and up to 73% for CLV1, with heritable edits recovered in progeny. The study involved Cas9-expressing plants, with results including fully albino seedlings and plants with increased floral organ number.

Use Cases

• Analyze somatic editing frequencies (80-95% for PDS, up to 73% for CLV1) to compare gene targeting efficiency.
• Study heritability patterns by examining the recovery of edited progeny from infected T0 plants.
• Investigate phenotypic outcomes like albinism from PDS frameshift mutations and increased floral organ number from CLV1 edits.
• Examine editing robustness in a tetraploid context by assessing mono-, bi-, tri-, and tetra-allelic mutations.

Strengths

• Reports high somatic editing frequencies with specific percentages (80–95% for PDS, up to 73% for CLV1).
• Demonstrates heritable edits, with 100% of PDS-targeted plants (n=5) and 60% of CLV1-targeted plants producing edited progeny.
• Documents editing efficacy in a tetraploid genetic background, showing mutation across four alleles.

Limitations

• Data is presented in a PDF (45.5 KB), not a structured, machine-readable tabular format.
• Sample sizes for specific experiments are small (e.g., n=5 plants for PDS heritability).
• The primary Cas9-expressing line was tetraploid, which may not represent typical diploid editing scenarios.

Provenance

Source

figshare, author Redeat Tibebu.

Collection Method

Experimental study using Tobacco rattle virus (TRV) to deliver guide RNAs into Cas9-expressing groundcherry plants.

Freshness

Last updated 2026-03-20.