Bovine Sperm Proteomics Before and After Cryopreservation from 5 Bulls

A proteomic dataset comparing protein abundance in bovine spermatozoa before and after cryopreservation. The study identified 1,373 proteins and found 84 with significant differential abundance between fresh and cryopreserved states. The data was generated by Alexandra Keller using LC-MS/MS analysis and published on figshare in 2026.

Use Cases

• Train classification models to predict sperm cryosurvival based on the 84 differentially abundant proteins.
• Identify protein biomarkers for mitochondrial integrity and redox homeostasis as mentioned in the functional analysis.
• Analyze patterns in protein co-expression related to cytoskeletal organization and metabolic processes.
• Validate proteomic signatures associated with apoptosis-like and capacitation-like changes described in the study.

Strengths

• Includes 1,373 identified proteins, providing a broad proteomic profile.
• Based on samples from five sexually mature bulls with proven fertility.
• Differential analysis identified 84 proteins with significant abundance changes (log2FC > 2, p < 0.05).
• Functional enrichment analysis links protein changes to specific biological pathways.

Limitations

• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The dataset is small (23.0 KB), indicating limited scope, likely containing summary results rather than raw spectra.
• Data may reflect bias inherent to the specific experimental protocol and bull cohort used.

Provenance

Source

figshare

Collection Method

Bottom-up proteomic approach using LC-MS/MS analysis on semen samples divided into fresh and cryopreserved groups.

Time Range

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Freshness

Last updated 2026-04-16 05:26:12.

Geography

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