ant(9)-Ie: Novel Spectinomycin Resistance Gene Characterization in Providencia

A novel chromosomally encoded aminoglycoside resistance gene, ant(9)-Ie, was identified in Providencia rettgeri P17 from animal farm wastewater. The recombinant strain showed a 64-fold increase in spectinomycin MIC, with the enzyme's catalytic efficiency measured at (8.22 ± 1.24) × 10² M⁻¹·s⁻¹. Lulu Huang published this research data on figshare in 2026.

Use Cases

• Comparative genomic analysis based on the conserved genetic environment of ant(9)-Ie homologous genes.
• Kinetic modeling of enzyme activity based on the reported spectinomycin catalytic efficiency (kcat/Km).
• Phylogenetic analysis of resistance gene backgrounds based on the reported amino acid sequence similarity to ANT(9)-Id (58.67%).
• Assessing clinical relevance of novel resistance genes based on the source analysis of Providencia strains in NCBI (mostly human clinical specimens).

Strengths

• Includes specific kinetic parameter for the novel enzyme: spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Reports a concrete experimental result: a 64-fold increase in the Minimum Inhibitory Concentration (MIC) of spectinomycin for the recombinant strain.
• Data is associated with a specific, novel biological discovery: the ant(9)-Ie gene.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 16.3 KB file size suggests a very limited data scope, likely containing summary results rather than raw experimental data.

Provenance

Source

Lulu Huang via figshare.

Collection Method

Data likely generated from bacterial isolation, molecular cloning, heterologous expression, kinetic analysis, and whole-genome sequencing as described.

Freshness

Last updated 2026-06-02 04:28:47; freshness should be verified.