Genomic Determinants of Antifungal Activity in Streptomyces Strain STM-2

Genomic analysis results for Streptomyces melanosporofaciens STM-2, a strain isolated from Chiayi County, Taiwan. The analysis identified 52 biosynthetic gene clusters (BGCs) and specific genes for antifungal enzymes. It was created by Muhammed Opoku Gyamfi and last updated in March 2026.

Use Cases

• Identify and categorize the 52 biosynthetic gene clusters (BGCs) predicted by antiSMASH, including type I/II PKS, NRPS, and hybrid systems.
• Analyze the accessory genome containing unique genes associated with specialized antifungal activity, such as chitinases and β-1,3-glucanases.
• Examine the high-GC (71%) linear chromosome structure and its annotated features for comparative genomics studies.
• Use the in silico structural models generated by AlphaFold2, which have high pLDDT scores, for functional analysis of unique gene products.

Strengths

• Genome mining predicted an extensive repertoire of 52 biosynthetic gene clusters (BGCs).
• Comparative analysis was supported by high-resolution Average Nucleotide Identity (ANI) and digital DNA–DNA hybridization (dDDH) scores.
• In silico structural analysis using AlphaFold2 yielded three-dimensional protein models with very high pLDDT scores.

Limitations

• The dataset is very small at 12.1 KB, indicating limited scope, likely containing summary results rather than raw genomic sequences.
• The specific data structure (rows, columns) and sample data are unavailable, making it difficult to assess the format and granularity of the information.
• The analysis is focused on a single novel strain (STM-2), limiting generalizability to other Streptomyces species.

Provenance

Source

figshare, authored by Muhammed Opoku Gyamfi.

Collection Method

Integrated approach involving physiological/biochemical characterization, genome sequencing, genome mining with antiSMASH, comparative pangenomic analysis, and in silico structural modeling with AlphaFold2.

Freshness

Last updated on 2026-03-20.

Geography

Chiayi County, Taiwan (source of the isolated STM-2 strain).