Integrated Transcriptome and scRNA-seq Data for Ulcerative Colitis Biomarker Discovery

Three biomarkers (ARHGEF3, S100A8, and RHOU) were identified for ulcerative colitis diagnosis using integrated transcriptomic data and machine learning. The supporting data, uploaded by Meilin Chen on April 22, 2026, is a 20.5 KB Excel file containing results from a study that validated these biomarkers across multiple independent cohorts. A diagnostic nomogram built from these markers achieved an AUC of 0.991 in the training set.

Use Cases

• Validate diagnostic biomarkers for ulcerative colitis based on the identified genes ARHGEF3, S100A8, and RHOU.
• Investigate immune cell infiltration patterns in ulcerative colitis based on the 27 differentially expressed immune cells mentioned.
• Study macrophage metabolic reprogramming in ulcerative colitis using the cross-dataset single-cell RNA sequencing analysis referenced.
• Build diagnostic models to differentiate ulcerative colitis from Crohn's disease based on the discriminative potential of the identified biomarkers.

Strengths

• Biomarkers were validated across multiple independent external cohorts, with a diagnostic nomogram achieving an AUC of 0.968 in one validation set.
• The study employed four machine learning algorithms for biomarker screening and integrated cross-dataset single-cell RNA sequencing for mechanistic investigation.
• Expression levels of the three biomarkers significantly correlated with clinical severity and pathological mucosal states.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 20.5 KB, indicating it likely contains summary or processed results rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Integrated from multiple transcriptomic datasets and validated with single-cell RNA sequencing and in vivo experiments.

Freshness

Last updated 2026-04-22 05:49:15; freshness should be verified.