Lactylation-Related Gene Biomarkers for Acute Myeloid Leukemia Prognosis

Seven hub lactylation-related genes (LRGs) were identified as prognostic biomarkers for acute myeloid leukemia (AML). The dataset includes genes LSP1, MPO, GZMB, SPINK2, HLA-DRB1, HLA-DRA, and POU2F2, with GZMB and LSP1 validated via Mendelian randomization. Analysis leveraged data from GEO and TCGA databases, applying machine learning algorithms like LASSO-logistic and SVM-RFE for gene selection.

Use Cases

• Train a GLM model to predict AML prognosis using the seven hub LRGs (LSP1, MPO, GZMB, SPINK2, HLA-DRB1, HLA-DRA, POU2F2) as features.
• Analyze the relationship between immune cell infiltration and key LRGs like GZMB and LSP1 using CIBERSORT.
• Perform molecular docking to screen potential drugs targeting the GZMB and LSP1 gene products.
• Validate the prognostic power of the seven hub LRGs using univariate COX regression and machine learning algorithms (LASSO-logistic, SVM-RFE, Boruta).

Strengths

• Identifies seven specific hub lactylation-related genes (LSP1, MPO, GZMB, SPINK2, HLA-DRB1, HLA-DRA, POU2F2) with clear biological relevance to AML.
• Validation of two genes (GZMB and LSP1) via two-sample Mendelian randomization analysis strengthens causal inference.
• Analysis integrates multiple data sources (GEO, TCGA) and methodologies (single-cell sequencing, WGCNA, machine learning).

Limitations

• The dataset is small in file size (417.3 KB), indicating limited raw data volume, likely containing summary results rather than primary sequencing data.
• Specific row and column counts for the underlying data tables are unknown, limiting assessment of statistical power.
• Findings are specific to acute myeloid leukemia and the identified lactylation-related genes, limiting generalizability to other cancers.

Provenance

Source

Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases.

Collection Method

Computational analysis of RNA and single-cell sequencing data using Seurat, limma, WGCNA, and machine learning algorithms.

Freshness

Last updated March 23, 2026.