Mouse Duodenal Transcriptome and Microbiome Data for Ochratoxin A Ferroptosis Study

A mouse study dataset from figshare, authored by Shaokat Ali and last updated in April 2026. It contains integrative transcriptomic and 16S rRNA microbiome data from mice exposed to Ochratoxin A for seven days. The data includes measurements of 769 differentially expressed genes, serum LPS, duodenal MDA, iron levels, and antioxidant defenses.

Use Cases

• Identify ferroptosis-related gene expression patterns based on the 769 differentially expressed genes (DEGs) and 26 overlapping ferroptosis-regulating genes.
• Analyze gut microbiome dysbiosis based on 16S rRNA sequencing data showing altered Bacteroidota abundance and pathogenic enrichment.
• Correlate biochemical markers like malondialdehyde (MDA) and iron levels with histological damage indicators described in the study.
• Investigate pathways linking redox imbalance and iron homeostasis based on the enrichment of key downregulated genes (SLC7A11, GSTM1, CP, SLC40A1) in ROS/lipid metabolism.

Strengths

• Integrates multiple data types: transcriptomics (769 DEGs), 16S rRNA microbiome profiling, and biochemical assays (MDA, iron, LPS).
• Study design is clearly described, including a 7-day exposure to a specific OTA dose (2 mg/kg body weight/day).
• Published under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is very small (4.1 KB), suggesting it may contain summary or processed results rather than raw sequencing data.
• Data is from a single controlled mouse study, which may limit generalizability.

Provenance

Source

figshare, author Shaokat Ali.

Collection Method

Data generated from a mouse exposure study using transcriptomics, qRT-PCR, Western blotting, immunofluorescence, biochemical assays, and 16S rRNA sequencing.

Time Range

Experimental period covered seven days of exposure.

Freshness

Last updated 2026-04-13 04:35:15; freshness should be verified.

Geography

Laboratory study; geographic scope is not specified.