Melanoma Stem Cell and Macrophage Interaction Data

Melanosphere cultures from A375 and WM115 cell lines were used to model how melanoma stem cells reprogram macrophages. RNA-seq analysis revealed stem cell-conditioned media induced a non-canonical macrophage phenotype enriched for inflammatory and immune-regulatory programs. A derived macrophage score was associated with shorter overall survival in an independent melanoma patient cohort.

Use Cases

• Analyzing macrophage transcriptomic reprogramming based on RNA-seq data from stem cell-educated macrophages.
• Investigating monocyte migration patterns in response to stem cell-conditioned media and CCR2 inhibition.
• Correlating a stem cell-educated macrophage gene signature with clinical outcomes like overall survival in melanoma cohorts.
• Studying the effect of macrophage-conditioned medium on NK-cell cytotoxicity against melanoma cells.
• Examining cell line-dependent changes in stemness-associated transcriptional regulators following macrophage exposure.

Strengths

• Dataset is associated with a detailed experimental methodology described across all sources, including specific cell lines (A375, WM115) and protocols.
• Includes exploratory clinical validation, linking in vitro findings to patient survival data in an independent melanoma cohort.
• Available under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Column names and row counts are not provided in any source, limiting understanding of the data's structure and scale.
• Two different file sizes are reported (828541 and 13222), suggesting possible multiple files or a conflict in metadata, but their specific contents are not detailed.
• Metadata is sparse, lacking information on the author's organization and the specific variables contained within the Excel file.

Provenance

Source

Martina Anselmi

Collection Method

Data likely generated from in vitro experiments using melanosphere cultures, THP-1 monocytes, RNA-seq, and analysis of a clinical melanoma cohort.

Freshness

Last updated on 2026-06-02.