NGF Administration Effects on GnRH Phenotype in Aging Mouse Hypothalamic Stem Cells

702.7 KB of experimental data from a study on nerve growth factor (NGF) administration in male senescence-accelerated mouse P8 (SAMP8) models. The dataset, authored by Jiao Luo and last updated on 2026-04-24, likely contains results from serum hormone measurements, immunofluorescence analyses, and transcriptomic profiling related to hypothalamic-pituitary-testicular axis function.

Use Cases

• Analyze the relationship between NGF dosage and serum hormone levels (LH, FSH, testosterone) based on time-series measurements mentioned in the description.
• Investigate cellular phenotype changes based on immunofluorescence data for GnRH immunoreactivity and Sox2/GnRH co-localization in the OVLT.
• Study in vitro differentiation pathways based on transcriptomic profiling and qPCR data from hypothalamic neural stem cell cultures.
• Model the effects of kisspeptin signaling modulation based on experimental results using kisspeptin agonists and antagonists.

Strengths

• Data is associated with a detailed methodological description including specific NGF dosages (6.25, 12.5, 25 μg/kg) and multiple post-injection time points (8 h, 24 h, 96 h, 7 d, 10 d).
• The study employs multiple validation approaches, including in vivo immunofluorescence, in vitro 3D culture, and transcriptomic profiling.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (702.7 KB), indicating a limited scope of data.

Provenance

Source

figshare

Collection Method

Experimental data from a controlled laboratory study involving mouse models, immunofluorescence, hormone assays, and cell culture.

Freshness

Last updated 2026-04-24 05:40:50; freshness should be verified.