Pharmacological and Genetic Modulation of IL-32 Expression in Intestinal Epithelial Cells

Experimental data from a study investigating the role of IL-32 in HIV-1 latency and reactivation at the intestinal barrier. The dataset, published by Etiene Moreira Gabriel on figshare in 2026, includes results from cytokine treatments, CRISPR/Cas9 gene editing, and viral outgrowth assays using cells from ART-treated people with HIV-1. Findings indicate that modulating IL-32 expression in intestinal epithelial cells does not significantly impact HIV-1 outgrowth from co-cultured CD4+ T-cells.

Use Cases

• Analyzing the effects of cytokine treatments (IL-22, ATRA, RGZ) on IL-32 isoform expression based on RT-PCR and ELISA data.
• Investigating transcriptional changes in intestinal epithelial cells following IL32 knockout using RNA-Sequencing results.
• Evaluating the impact of epithelial cell activation on HIV-1 outgrowth from CD4+ T-cell reservoirs based on viral outgrowth assay (VOA) and p24 measurements.

Strengths

• Data is associated with a detailed, peer-reviewed-style methodology described in the background and methods sections.
• The dataset is licensed under CC-BY-4.0, permitting broad reuse with attribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 3.8 MB file size suggests a relatively small dataset, potentially limiting statistical power for some analyses.

Provenance

Source

figshare, authored by Etiene Moreira Gabriel.

Collection Method

Laboratory experiments using HT-29 intestinal epithelial cells, CRISPR/Cas9 gene editing, RT-PCR, ELISA, RNA-Sequencing, and viral outgrowth assays with cells from ART-treated people with HIV-1.

Freshness

Last updated 2026-05-26 07:48:56; freshness should be verified.