Antibody CDR3-VH Repertoire Physicochemical Signatures for PS-ASO Recognition

Riccardo Galasso published this dataset on 2026-05-29. It contains physicochemical descriptor profiles for 113 shared CDR3-VH clonotypes identified from phage display selections against phosphorothioate-modified oligonucleotides. The data was generated via Illumina MiSeq sequencing and a dedicated bioinformatic pipeline for clonotype reconstruction and analysis.

Use Cases

• Identify convergent physicochemical signatures in antibody sequences based on the described positive charge, aromatic residue patterns, and pI profiles.
• Train models to predict antibody-antigen binding specificity using the described CDR3-VH length distribution and hydrophobicity features.
• Benchmark repertoire analysis pipelines using the NGS-guided framework and enrichment analysis results described.
• Prioritize candidate antibody binders for experimental validation based on the shared clonotype set and associated functional assay data mentioned.

Strengths

• Data is derived from deep sequencing on an Illumina MiSeq platform, suggesting a high-throughput experimental source.
• Analysis identifies a defined set of 113 shared CDR3-VH clonotypes with reproducible enrichment.
• Dataset is licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 15.0 KB, indicating limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare, author Riccardo Galasso.

Collection Method

Generated via a next-generation sequencing-guided phage display strategy, coupled with a dedicated bioinformatic pipeline for clonotype reconstruction.

Freshness

Last updated 2026-05-29 04:54:24; freshness should be verified.