Proteomic Analysis of Lysinibacillus PET Biodegradation Potential

12.9 KB Excel file contains proteomic data from a study on a PET-associated bacterial strain isolated from Icelandic soil. The dataset compares the early-stage growth of Lysinibacillus sp. against Ideonella sakaiensis over six weeks, with proteomic analysis revealing oxidative-stress response proteins rather than canonical PET-degrading enzymes. Author Radoslaw B. Dudziak published the data in 2026 under a CC BY 4.0 license.

Use Cases

• Analyze proteomic profiles to identify oxidative-stress response proteins like peroxiredoxins and superoxide dismutases expressed under PET exposure.
• Compare early-stage growth assay data between Lysinibacillus sp. and Ideonella sakaiensis across six weeks to assess microbial adaptation strategies.
• Examine in silico screening results for the absence of PETases or hydrolases with the GXSXG motif or Ser-His-Asp catalytic triad.
• Investigate FE-SEM surface imaging data for evidence of polymer surface alterations such as erosion, fissures, and delamination.

Strengths

• Data originates from a controlled scientific study integrating growth assays, proteomics, in silico screening, and surface imaging.
• Proteomic analysis specifically targeted the absence of known PET-degrading enzymes, providing a clear negative result.
• Comparative analysis includes a reference strain (Ideonella sakaiensis) with statistically significant growth differences reported (p < 0.05 at multiple weeks).

Limitations

• The dataset is very small at 12.9 KB, indicating limited scope and likely a summary table rather than raw experimental data.
• Proteomic analysis did not detect any canonical PET-degrading enzymes, limiting its utility for studying direct enzymatic depolymerization.
• The sample source is specific to a single bacterial strain from Icelandic soil, limiting generalizability.

Provenance

Source

Radoslaw B. Dudziak via figshare.

Collection Method

Proteomic characterization via MALDI-TOF MS, growth assays, in silico genome-wide screening, and FE-SEM surface imaging of a bacterial strain isolated from soil.

Time Range

Experimental period covers six weeks of growth assays.

Freshness

Last updated in March 2026.

Geography

Bacterial strain isolated from Icelandic soil.