YWHAZ RNA-Seq and iRIP-Seq Data for Diabetic Foot Ulcer Analysis

An Excel dataset containing results from a study on the RNA-binding protein YWHAZ in diabetic foot ulcer (DFU) tissues. The data includes 1,072 differentially expressed genes from RNA-seq and 57 selectively bound DEGs from integrated iRIP-seq and RNA-seq analyses. The dataset was authored by Tianjian Zha and last updated on 2026-05-07.

Use Cases

• Identify gene expression changes associated with YWHAZ knockdown based on the 1,072 differentially expressed genes.
• Analyze RNA-protein binding interactions based on iRIP-seq results showing YWHAZ binding to GC-rich motifs.
• Investigate pathways like insulin resistance and insulin signaling based on the enrichment of the SREBF1 gene.
• Study the role of specific genes (e.g., AREG, FOSL1, LAMB3, COL12A1) in cell proliferation, migration, and adhesion in DFU.

Strengths

• Includes results from multiple experimental methods: RNA-seq, RT-qPCR, immunohistochemistry, and iRIP-seq.
• Identifies a specific set of 57 differentially expressed genes that were selectively bound by the YWHAZ protein.
• Validated YWHAZ upregulation in DFU tissues through independent public RNA-seq data and lab techniques.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 9.7 KB, indicating limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare, author Tianjian Zha.

Collection Method

Generated from laboratory experiments including RNA-seq and improved RNA immunoprecipitation (iRIP)-seq on DFU tissues and HaCaT cells.

Freshness

Last updated 2026-05-07 04:47:34; freshness should be verified.