Temporal Transcriptional Dynamics in a Mouse Model of Cutaneous Leishmaniasis

A longitudinal transcriptomic dataset characterizes host responses to Leishmania braziliensis infection in a BALB/c mouse ear dermal model. The data includes bulk RNA sequencing from skin lesions and draining lymph nodes at six time points from 2 hours to 77 days post-infection. The dataset was authored by Jessica Lobo-Silva and published on figshare in May 2026.

Use Cases

• Identify stage-specific gene expression modules based on the described early innate (M3), tissue damage (M1), and skin barrier remodeling (M6) responses.
• Analyze conserved epigenetic regulators and microRNAs for cross-species comparison based on the 16 conserved microRNAs and 12 shared epigenetic regulators mentioned.
• Investigate macrophage polarization and nitric oxide metabolism pathways based on the upregulation of genes like Il12a, Il12b, Il4, Arg1, and Nos2.
• Study the dynamics of long non-coding RNAs based on the described module (M2) selectively downregulated during ulceration.

Strengths

• Provides a longitudinal framework with data from six specific time points (2h, 6h, 48h, 14d, 35d, 77d post-infection).
• Enables cross-species validation, as ulcerated mouse lesions recapitulated 77% of inflammatory pathways described in human CL.
• Identifies specific actionable targets, including 16 conserved microRNAs and 12 shared epigenetic regulators.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (414.2 KB), indicating a limited scope likely focused on summary or differential expression results rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Bulk RNA sequencing performed on skin lesions and draining lymph nodes from a BALB/c mouse model infected with Leishmania braziliensis.

Time Range

Data covers a longitudinal study from 2 hours to 77 days post-infection.

Freshness

Last updated 2026-05-12 04:20:14; freshness should be verified.